Different outcomes were used to examine the impact of BCAA supplementation among athletes. The overview of the International Olympic Committee did not mention BCAAs in the different topics discussed—namely, supplements used to prevent or treat nutrient deficiencies, supplements used for energy provision, and supplements that improve sports performance . Additionally, the Australian Institute of Sport classified BCAAs in group C, which include supplements without scientific support among athletes or inconclusive studies. However, oral ingestion of BCAAs is highly questionable with reference to optimizing performance and protein synthesis 6,9,10. Moreover, most of the studies did not report the total protein intake across the day and, consequently, the benefits of BCAAs should be interpreted with caution. The protocols of BCAA supplements differed considerably between studies. Although BCAA oxidation occurs mainly in the muscle, the impact of carbohydrates on the muscle is more evident. Ratings of perceived exertion and mental fatigue were significantly reduced in the BCAA group among seven endurance cyclists , while in 50 marathon runners no differences were reported in ratings of perceived exertion . In endurance sports (running or cycling), the results of extracted studies are inconclusive. Risk of bias for each study 20,21,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43 using the revised Cochrane risk of bias tool for randomized trials. On the fourth domain—concerning measurement outcome—five studies demonstrated a higher risk of bias 32,38,39,41. Participant groups and blinding—Group 1 (BCAA supplementation) consisted of 50 subjects (25 men and 25 women) who supplemented their diet with BCAAs. Both groups followed the same training program designed to improve physical performance and body composition. One-repetition maximum (1 RM) was measured for each exercise before training and 3 d after the last training session to evaluate strength changes. All training sessions were supervised by a study researcher (M.F.) to ensure correct technique and adherence to the prescribed exercise intensity. The effects of BCAAs on performance were negligible irrespective of short or long-term protocols of supplementation. The effects of BCAAs on recovery and [tvoyaskala.com](https://tvoyaskala.com/user/startevent6/) perceived muscle soreness were largely dependent of sport and exercise modality. The cortisol level decreased after exercise and, in parallel, [buy testosterone gel online](https://hack.allmende.io/s/hxM8dwkML) tended to rise in participants that engaged in resistance training . Two studies reported that the consumption of BCAA supplementation attenuated the decrements in performance 36,37.. Nevertheless, BCAAs are often combined with other macronutrients or supplements and this issue needs future research.} Then, randomization was performed with a computer-based random number generator resulting in two groups (BCAA supplementation and placebo) with equal numbers of men and women and balanced initial fitness levels. The randomization process for this study was performed to ensure that each participant had an equal chance of being assigned to either the BCAA supplementation or placebo group, thereby minimizing potential bias and confounding variables. We also excluded participants who were currently taking dietary supplements, as this could have influenced their response to the BCAA supplementation used in the study. By examining these factors in both male and female participants, this study seeks to provide insights into how BCAA supplementation can be optimized for different sexes, ultimately contributing to more effective and personalized sports nutrition strategies. In several studies involving endurance athletes , recreational runners , and cyclists , [pads.jeito.nl](https://pads.jeito.nl/s/qoEY4PKu4p) comparable changes in body weight were observed in BCAA and control groups after 7 days of oral supplementation . Studies evaluating the impact of BCAAs on performance suggest that supplementation had insignificant effects on performance outcomes in cyclists and runners 25,26,27,28, volleyball players , and soccer players 30,31, although variation in supplementation protocols must be taken into account.